P105 Epithelial microRNA signatures as potential biomarkers in Crohn’s disease related to clinical and endoscopic activity

Abstract Background Emerging evidence suggests that epithelial cell-derived microRNAs (miRNA) are involved in the pathogenesis of inflammatory bowel disease; however, as now specific implications real clinical practice, especially relation to biologics use yet far-fetched. Therefore, we tried investigate cell derived miRNA signatures related and endoscopic activity Crohn’s disease (CD) patients. Methods We prospectively collected terminal ileum tissue samples via ileocolonoscopy from healthy control (n=12), CD patients remission (n=32) active (n=36). was defined Crohn's index (CDAI) <150 achievement mucosal healing. Both inflamed non-inflamed lesions were sampled Clinical information, medication use, chemistry data measured within a week sample date. levels by small-RNA sequencing qRT-PCR. Diagnostic ability found evaluated receiver operating characteristic (ROC) curve analysis. Results The study cohort followed up until January 2022. Mean follow-up period prospective 13.06 months. In comparing control, state revealed profiles sequencing. Through RT-PCR validation, expression miR-141-3p, miR-338-3p, miR-378a-3p, miR-200b-3p significantly decreased, while miR-125b-5p, miR-29b-3p miR-155-5p increased Moreover, case patients, expressions miR-150-5p, down-regulated those using biologics, compared with naïve This may suggest these miRNAs not only biomarkers but also inflammation response. ROC analysis suggested different diagnostic both status. MiR-338-3p, miR-141-3p miR-378a-3p demonstrated area under greater than 0.9 predicting Conclusion report identifying its suggesting potential treatment target pathogenesis. These could be positioning which intend explore near future.